Shiping Zhang

 

shipingphoto

 

Background & Contact Information

Position Postdoctoral Fellow (2016 – Present)
Education: Ph.D. 2016, Biochemistry and Molecular Biology, Tongji University (Advisor: Lei Xue)
Fellowships & Awards:
Email: shiping@email.unc.edu
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Research Information

The canonical Wnt signaling is an evolutionarily conserved pathway that regulates a wide range of physiological functions, including embryogenesis, organ maintenance, cell proliferation and cell fate decision. Dysregulation of Wnt signaling has been implicated in various cancers–most strikingly, mutations in Adenomatous polyposis coli (APC), a key negative regulator of Wnt signaling, initiate 80% of sporadic colon cancers.

Activation of Wnt signaling depends on the intracellular levels of the transcriptional co-activator β-catenin (β-cat). When Wnt signaling is off, β-cat is phosphorylated by a multi-protein destruction complex that includes APC and Axin, as well as the kinases GSK3 and CK1. This creates a binding site for the SCFβTrCP E3-ligase, which ubiquitinates β-cat and transfers it to the proteasome for degradation. When Wnt signaling is activated through the binding of a Wnt ligand to its receptor complex, destruction complex function is inhibited and β-cat levels rise, which allows β-cat to enter to the nucleus, bind with Tcf/Lef transcription factors and drive the expression of Wnt target genes.

Despite the importance of the destruction complex in maintaining low levels of β-cat, the principle function of each core component yet remains largely unknown. I am using Drosophila genetics and cell biology and cultured mammalian cells to investigate how Axin and APC interact, and their mechanisms of regulating Wnt signaling.

Publications
  1. Schaefer, K.N., Bonello, T.T.*,  Zhang, S.*, Williams, C.E., Roberts, D.M., McKay, D.J, and Peifer, M.  (2018).  Supramolecular assembly of the beta-catenin destruction complex and the effect of Wnt signaling on its localization, molecular size, and activity in vivo.  PLoS Genetics doi.org/10.1371/journal.pgen.1007339.  *=contributed equally and listed alphabetically.
  2. Shiping Zhang, Xiaowei Guo, Changyan Chen, Yujun Chen, Jikai Li, Ying Sun, Chenxi Wu, Yang Yang, Cizhong Jiang, Wenzhe Li*, Lei Xue*, dFoxO promotes Wingless signaling in Drosophila, Scientific Reports, 2016, 6: 22348.
  3. Xianjue Ma*, Yujun Chen, Shiping Zhang, Wenyan Xu, Yingyao Shao, Yang Yang, Wenzhe Li, Maoquan Li*, Lei Xue*, Rho1-Wnd signaling regulates loss-of-cell polarity-induced cell invasion in Drosophila. Oncogene, 2016, 35(7): 846-855.
  4. Shiping Zhang, Changyan Chen, Chenxi Wu, Yang Yang, Wenzhe Li, Lei Xue*, The canonical Wg Signaling modulates Bsk-mediated cell death in Drosophila, Cell Death & Disease, 2015, 6: e1713.
  5. Chenxi Wu#, Yujun Chen#, Feng Wang, Changyan Chen, Shiping Zhang, Chaojie Li, Wenzhe Li, Shian Wu, Lei Xue*, Pelle modulates dFoxO-mediated cell death in Drosophila, PLoS Genetics, 2015, 11(10) : e1005589.
  6. Shiping Zhang and Lei Xue. Progress on cell lineage analysis in Drosophila melanogaster. Hereditas (Beijing), 2012, 34(7): 819-828.
  7. Wenzhe Li, Senlin. Li, Hongyu. Zheng, Shiping Zhang, Lei Xue*, A broad expression profile of the GMR-GAL4 driver in Drosophila melanogaster, Genetics and Molecular Research, 2012, 11(3): 1997-2002.

 

(# Equal contribution, * Corresponding author)

 

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